A new extraction method for Acinetobacter species ODB-L2 rough form lipopolysaccharide from culture broth
Kunii, K.; Nakamura, S.; Sato, C.; Fukuoka, S.
Microbios 105(412): 153-161
2001
ISSN/ISBN: 0026-2633 PMID: 11414500 Document Number: 528438
The Gram-negative bacterium Acinetobacter species ODB-L2 produces lipopolysaccharide (LPS) in culture broth. The LPS could not be purified by conventional extraction methods using 90% phenol/water or 90% phenol/chloroform/petroleum ether mixed solvent. Extraction was achieved employing an admixture of chloroform, ethanol, and 4 M HCl solution. The LPS was purified from dissolving the crude extracts in 90% phenol and LPS sediment formed by addition of methanol. The LPS was characterized by chemical, biochemical, and physicochemical methods as rough form 3-hydroxydodecanoic acid rich LPS.