Effects of Buyang huanwu decoction on the release of vWF and the expression of tissue factor induced by tumor necrosis factor alpha in cultured bovine aortic endothelial cells
Shang, G.P.; Wen, Z.B.; Li, J.C.
Hunan Yi Ke da Xue Xue Bao 25(2): 129-131
2000
ISSN/ISBN: 1000-5625 PMID: 12212197 Document Number: 525781
To study the effects of Buyang huanwu decoction (BHD) on the release of von Willebrand factor(vWF) and the expression of tissue factor(TF) activity induced by tumor necrosis factor alpha (TNF alpha) in cultured bovine aortic endothelial cells (BAECs). BAECs from neonatal cox were cultured and 4-8 passages were used. Cells were incubated for 8 hours after addition of different treatments. The supernatant was used to measure vWF and BAECs lysate to determine TF activity. 1. Compared with the control, TNF alpha enhanced the expression of TF activity(12.79 +/- 2.59 vs. 4.69 +/- 0.83, P < 0.01) and the effects were in dose-dependent manner(r = 0.9712, P < 0.01); BHD inhibited the effects of TNF alpha(P < 0.01). 2. Compared with the control, TNF alpha promoted the release of vWF from endothelial cells (13.28 +/- 4.76 vs. 6.42 +/- 2.84, P < 0.01) and BHD inhibited the effect of TNF alpha(P < 0.01). BHD can inhibit the expression of TF and the release of vWF induced by TNF alpha.