A closer look at the insertion within helix 54 of the 23S-rRNA of the genus Corynebacterium (and related taxa)
Hügle, B.; Weizenegger, M.; Hengstler, K.; Fahr, A.; Bartel, J.
Clinical Laboratory 46(5-6): 255-260
2000
ISSN/ISBN: 1433-6510 PMID: 10853233 Document Number: 525297
Direct sequencing of the insertion in helix 54 of the 23S-rRNA of different strains of 8 species of the genus Corynebacterium was performed in order to determine the extent of variations of these strains. The results demonstrated that there is considerable variation within different strains of a single species. While this clearly jeopardizes the usefulness of the insertion with regard to species-specific diagnostic probes, the variations were found to concentrate within two clearly defined regions comprising the ascending and descending parts of the distal helix of a putative stemlike secondary structure. The remainder of the insertion sequences seem to be fairly constant within a single species while displaying considerable differences toward sequences of other species of the same genus.