Characteristics of an established cervical carcinoma cell line HCE1
Wu, S.F.; Zhu, H.C.; Gu, F.H.; Huang, B.Y.; Liu, H.Y.
Hunan Yi Ke da Xue Xue Bao 25(6): 532-534
2000
ISSN/ISBN: 1000-5625 PMID: 12516395 Document Number: 520205
This paper deals with a new cell line established from 5 carcinoma specimens of hysterectomy and designated as HCE1. This cell line has been passaged more than 80 times(more than 18 months). It has the characteristics: 1. The pathological features of the tumor transplanted in nude mice were similar to that of the original inoculated lesion; 2. Electromicroscopically, there were rich microvilli on the surface of the cells. Abundant ribosomes were also observed; 3. The growth curves of the 25th passage were determined, the population doubline time calculated in the log phase of growth was 45.8 h; 4. The cloning efficiency in soft agar averaged 14.8% after counting 6 plates; 5. Karyo-type analysis showed aneuploidy with the modal chromosomal number 64-97.