Isolation, identification and certain characteristics of a Hantaan virus proposed as candidate virus for killed vaccine
Xie, Y.; Hang, C.; Wang, H.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 14(1): 27-30
2000
ISSN/ISBN: 1003-9279 PMID: 11503020 Document Number: 518683
Isolation and identification of a Hantaan virus from HFRS patient's serum for killed vaccine development. This virus temporally called FT14 detected by IFAT, RT-PCR. Partial sequence of M segment was determined by Sanger' a dideoxy chain termination method. The antigenicity and PCR amplification of the newly isolated virus FT14 proved that it belongs to virus of HTN type. Antiserum to the virus can neutralize many strains of Hantaan virus from different areas. The partial sequence of M segment of FT14, as compared with other HTN strains, showed great differences among nucleotide sequences, while the deduced amino acid sequence is comparatively conservative. The titer of the virus reached its peak at 9 days post inoculation in vitro, then the peak titer could last for a long time. This virus strain FT14 would be a good candidate virus for killed vaccine development.