Purification of follicle-stimulating hormone by radial-flow chromatography
Guo, L.A.; Yan, Z.; Yang, X.D.; Chu, X.Q.; Xu, H.F.
Se Pu 18(6): 577-579
2000
ISSN/ISBN: 1000-8713 PMID: 12541757 Document Number: 517275
By comparing the purification results of radial-flow chromatography with conventional chromatography, a new purification process of follicle-stimulating hormone (FSH) from crude sample of human menopausal gonadotrophin has been developed. The results showed that the specific activity, activity recovery, and purification factor of FSH purified by radial-flow chromatography were higher than those by conventional chromatography in large scale. The specific activity of FSH purified was 180 IU/mg protein, activity recovery 56%, purification factor 150. The purified product meets the quality requirement of the Standard of Chinese Pharmacopoeia.