The use of the fluorimetric method for studying DNA integrity exemplified by an assessment of dioxidine genotoxicity
Usol'tsev, M.V.; Durnev, A.D.; Seredenin, S.B.
Eksperimental'naia i Klinicheskaia Farmakologiia 63(2): 60-62
2000
ISSN/ISBN: 0869-2092 PMID: 10834098 Document Number: 516568
The genotoxic effect of dioxidine (300, 100, 30, and 10 mg/kg, i.p.) on the liver cells of outbred male rats was studied by fluorometric analysis of DNA unwinding (FADU) using the cell DNA samples taken from animals sacrificed 1, 6, 18, and 24 h after drug introduction. At a dose of 10 mg/kg, dioxidine produced no statistically significant increase in the amount of DNA damage relative to control. Increase in the dioxidine dose to 30 mg/kg resulted in a significant DNA damage 1 h after drug administration, while a dose of 100 or 300 mg/kg reliably caused damage at all times of exposure.