Fatty acids enhanced tubermycin production by Pseudomonas strain 2HS
Keudell, K.C.; Huang, J.K.; Wen, L.; Klopfenstein, W.E.; Bagby, M.O.; Lanser, A.C.; Plattner, R.D.; Peterson, R.E.; Weisleder, D.
Microbios 102(401): 27-38
2000
ISSN/ISBN: 0026-2633 PMID: 10817518 Document Number: 514221
A new microbial isolate, Pseudomonas 2HS, produced trace amounts of a greenish-yellow pigment when grown aerobically in a 1% yeast extract medium at 30 degrees C and shaken at 250 rpm for 5 days. In contrast, cells produced more greenish-yellow pigment (2.16 mg/15 ml culture) when grown in the presence of 0.5% 12-hydroxyoctadecanoic acid (w/v). The greenish-yellow pigment was identified as phenazine-1-carboxylic acid (tubermycin B), and the Pseudomonas 2HS was identified as P. aeruginosa 2HS. This is the first report that 12-hydroxyoctadecanoic, ricinoleic and other fatty acids can enhance the production of phenazine-1-carboxylic acid by a Pseudomonas species.