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The comparison of sensitivity between immunostaining and a simplified PCR-cold SSCP method in p53 genomic mutations

Ueno, I.; Ota, H.; Hidaka, E.; Ishikawa, M.; Hirota, M.; Nara, K.; Katsuyama, T.

Rinsho Byori. Japanese Journal of Clinical Pathology 48(5): 469-472

2000

ISSN/ISBN: 0047-1860
PMID: 10892297
Document Number: 513572
The PCR-SSCP (single strand conformational polymorphism) method has been widely employed to screen mutations in a variety of genes because of its rapidity and simplicity in the operation. Using this method, we have examined mutations of some tumor-related genes including p53 and Ki-ras. In this study, we have evaluated the PCR-Cold (non radioactive) SSCP method for detection of p53 point mutations in comparison with immunohistological detection of p53 and PCR-direct sequencing. The results indicated that the PCR-Cold SSCP method had the same sensitivity with that of PCR-direct sequencing method, and had higher sensitivity than that of immunohistochemical method (IHC).

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