Spectrophotometric determination of copper in the hair of the patients with rheumatoid arthritis
Cheng, X.Y.; Li, Z.H.
Hunan Yi Ke da Xue Xue Bao 25(2): 117-118
2000
ISSN/ISBN: 1000-5625 PMID: 12212193 Document Number: 512442
A new color reaction of copper (II) with solochrome cyanine R was studied by spectrophotometry. The results were that in the pH6 of HAc-NaAc buffer solution solochrome cyanine R reacted with copper (II) to form a violet complex; the maximum absorption of the complex was at 565 nm and its apparent molar absorption coefficient was 8.25 x 10(5) L.mol-1.cm-1. Beer's Law was obeyed in the range of 0-0.48 microgram.ml-1. This method was used to determine copper levels in the hair samples of 5 normal persons and 5 rheumatoid arthritis patients. The results showed that the hair copper level of arthritis patients was significantly higher than that of normal persons (P < 0.01).