O2 consumption and heart rate in developing zebrafish (Danio rerio) : influence of temperature and ambient O2
Barrionuevo, W.R.; Burggren, W.W.
American Journal of Physiology 276(2): R505-R513
1999
ISSN/ISBN: 0002-9513 PMID: 9950931 Document Number: 511490
Body mass, length, oxygen consumption (M O2) and heart rate (fH) were measured in "embryos" (prior to hatching), "larvae" (days 10-20), "juveniles" (days 30-70 in 10-day intervals), and "adults" (day 100) of the zebrafish Danio rerio. Fish were chronically reared at either 25, 28, or 31°C and then acutely exposed to hypoxia at different developmental stages. We hypothesized that at any given rearing and measurement temperature, D. rerio would maintain M O2 at lower ambient P O2 20 days in D. rerio and, thereafter, the ability to maintain M O2 in the face of ambient hypoxia progressively builds through to adulthood. Additionally, the temperature responses of metabolism and heart rate differ substantially at different phases of development, suggesting a loose coupling between the respiratory and cardiovascular systems, at least early in development. Reprinted by permission of the publisher.