Effects of hypoxia/reoxygenation and cytokines on adhesion of leukocytes to cerebral microvascular endothelial cells
Li, T.J.; Rui, Y.C.; Xu, J.P.
Zhongguo Yao Li Xue Bao 20(2): 137-140
1999
ISSN/ISBN: 0253-9756 PMID: 10437160 Document Number: 510628
AIM: To study the influence of hypoxia/reoxygenation (H/R) on the adhesion of neutrophils (Neu) and monocytes (Mon) to cultured bovine cerebral microvascular cells induced by tumor necrosis factor alpha (TNF-alpha) or interleukin 1-alpha (IL-1alpha). METHODS: Bovine cerebral microvascular endothelial cells (CMEC) and smooth muscle cells (CMSMC) were exposed to normoxia or H/R in the presence or absence of TNF-alpha (IL-1alpha). The number of adhesions of Mon and Neu to CMEC and CMSMC was measured by flow cytometry. RESULTS: CMEC were subjected to hypoxia for 2 h followed by reoxygenation with TNF-alpha (2 mug cntdot L-1) for 2 h, the adhesion rate of CMEC for Mon and Neu were increased to 35.0 % +- 0.9 % and 36.0 % +- 0.6 % (the TNF-alpha treated group was 28.9 % +- 1.1 % and 28.8 % +- 1.3 %), respectively. CMSMC were treated as above, the adhesion rate of Mon and Neu was enhanced to 49.9 % +- 0.4 % and 43.9 % +- 1.4 % (the TNF-alpha treated group was 34.0 % +- 1.9 % and 34.0 % +- 1.3 %), respectively. CMEC and CMSMC were treated by IL-1alpha, similar results were obtained. CONCLUSION: H/R enhanced the adhesion of Mon and Neu to CMEC and CMSMC induced by TNF-alpha or IL-1alpha.