Effects of growth factors on DNA and collagen synthesis of human mandibular condylar cartilage cells
Jiao, Y.; Wang, D.; Han, W.; Li, T.
Hua Xi Kou Qiang Yi Xue Za Zhi 17(4): 358-360
1999
ISSN/ISBN: 1000-1182 PMID: 12539387 Document Number: 510007
To investigate the effects of transforming growth factor-beta 1 (TGF-beta 1), insulin-like growth factor-I (IGF-I) and basic fibroblast growth factor(bFGF) on DNA and collagen synthesis of mandibular condylar cartilage(MCC) of human fetus. Cell culture, 3H-TdR and 3H-Proline incorporation methods were used. MCC cells were harvested from 4 to 5 months old human fetus. Cells were seeded at 2 x 10(4)/well on 96-well Plate. After synchronization, medium was replaced by DMEM containing 0.4% NCS with various growth factors and concentrations. bFGF stimulated the DNA synthesis significantly, and IGF-I had less effect, while the effect of TGF-beta 1 was insignificant. For collagen synthesis, bFGF caused a dose-dependent increase(60%). A greater effect(98%) was achieved when IGF-1 was added. In contrast, TGF-beta 1 could inhibit collagen synthesis (24%). Growth factors play an important part in the proliferation and matrix synthesis of MCC cells, which might be of potential application in treating cartilage destructive lesions.