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Preparative isolation of gonadotropin and urokinase on carboxylic cation exchangers

Demin, A.A.; Chernova, I.A.; Potapenko, B.E.; Toporkova, E.B.; Shataeva, L.K.

Prikladnaia Biokhimiia i Mikrobiologiia 35(4): 382-387

1999

ISSN/ISBN: 0555-1099
PMID: 10546278
Document Number: 509879
Low-pressure ion-exchange chromatography was used for sequential isolation of chorionic gonadotropin and urokinase (EC 3.4.99.26) from urine of pregnant women. Theoretical analysis of electrostatic interactions of protein macromolecules with the sorptive surface of a carboxylic cation exchanger was performed. This analysis allowed us to optimize the pH values for selective sorption and reversible desorption of the target component on a cation exchanger with a selected acidity of functional groups. The use of carboxylic cation exchangers KM-2p and Biokarb-GM allowed us to obtain the preparations with purity not inferior to commercially available preparations.

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