Validation of the LacTek test applied to spiked extracts of tissue samples: determination of performance characteristics
Mitchell, J.M.; Yee, A.J.; McNab, W.B.; Griffiths, M.W.; McEwen, S.A.
Journal - Association of Official Analytical Chemists 82(1): 79-84
1999
ISSN/ISBN: 0004-5756 PMID: 10028674 Document Number: 509176
LacTekTM tests are competitive enzyme-linked immunosorbent assays intended for rapid detection of antimicrobial residues in bovine milk. In this study, the LacTek test protocol was modified for use with extracts of bovine tissue to detect beta-lactam, tetracycline, and sulfamethazine residues. Test performance characteristics-precision, accuracy, ruggedness, practicability, and analytical specificity and sensitivity-were investigated. Results suggest that LacTek tests can be easily adapted to detect antimicrobial residues in extracts of lean ground beef. However, positive samples may not contain residues at violative concentrations (i.e., Canadian maximum residue limits), and therefore, additional analysis would be required for final confirmation and quantitation (e.g., chromatography).