Oligonucleotide arrays for high resolution HLA typing
Guo, Z.; Hood, L.; Petersdorf, E.W.
Reviews in Immunogenetics 1(2): 220-230
1999
ISSN/ISBN: 1398-1714 PMID: 11253948 Document Number: 508604
The methodology and applications of oligonucleotide array technology are reviewed. An oligonucleotide array system for typing HLA-B alleles is described. Oligonucleotide probes representing all known polymorphisms in exons 2 and 3 of HLA-B are immobilized on glass slides. Exons 2 and 3 are amplified from human genomic DNA using fluorescent-labeled primers and subsequently allowed to hybridize to an oligonucleotide array on glass supports. The presence of positive hybridization is detected by fluorescence scanning. HLA-B alleles are assigned by quantitative analysis of hybridization patterns. Proof of principle has been established in blinded testing of heterozygous DNA samples.