Use of high-density filters ("DNA arrays") for gene expression analysis in human tissues
Oddou, S.; Devilard, E.; Dales, J.P.; Hassoun, J.; Birg, F.; Xerri, L.
Annales de Pathologie 19(6): 557-561
1999
ISSN/ISBN: 0242-6498 PMID: 10617820 Document Number: 498537
Most technical strategies for the analysis of gene expression in tissues are able to study only one protein or RNA product at the same time. A new recent method referred to as <<DNA array>> or <<high density filter>> is able to analyze simultaneously several hundreds of different genes. The DNA array is a nylon membrane on which are spotted equal amounts of cDNAs corresponding to different genes. This filter is hybridized with a <> probe synthesized with mRNA derived from the tissue analyzed. The result gives a global profile of gene expression within the tissue and allows quantitative and comparative analysis between different tissues or cell types.