Inhibitory effects of 8- (N,N-diethylamino) -n-octyl-3,4,5-trimethoxybenzoate (TMB-8) on intracellular Ca2+ elevated by neurotransmitters in brain cells
Zhang, X.Q.; Wang, B.; Zhang, M.Y.; Xiao, J.G.
Zhongguo Yao Li Xue Bao 20(10): 893-896
1999
ISSN/ISBN: 0253-9756 PMID: 11270987 Document Number: 507746
To study the effects of TMB-8 on [Ca2+]i elevation induced by neurotransmitters in dissociated brain cells. The brain cell suspension was made using a gentle trituration for 1 min with a polished pipette. The changes of [Ca2+]i were detected by the fluorescent indicator, Fura 2-AM. In the presence of extracellular Ca2+ 1.3 mmol.L-1, sodium glutamate (Glu), histamine (His), and serotonin (5-HT) markedly increased the [Ca2+]i which were reduced by TMB-8 30 mumol.L-1. TMB-8 3 mumol.L-1 produced inhibitory effects on the increase of [Ca2+]i by His and 5-HT in a Ca(2+)-free Hanks' solution. The increase of [Ca2+]i by His and 5-HT was reduced to control level by TMB-8 10 mumol.L-1. TMB-8 inhibited the [Ca2+]i elevation induced by Glu, 5-HT, and His in brain cells.