Alterations of intracellular free calcium concentration of cerebral neuronal cells in infectious brain injury in rats
Chen, X.; Yang, Y.; Tao, Y.; Yu, P.; Chen, L.
Hunan Yi Ke da Xue Xue Bao 23(4): 353-355
1998
ISSN/ISBN: 1000-5625 PMID: 11189391 Document Number: 491289
Intracellular free calcium concentration, [Ca2+]i, of cerebral synaptosomes loaded by Fura-2/AM was measured by fluorescent method to clarify alterations of [Ca2+]i in infectious brain injury (IBI) in rats. The results showed that [Ca2+]i, water content (WC), and Evans blue content (EB) in groups injected by Bordetella pertussis suspension (BP) were increased compared to those in groups injected by normal saline (NS) at 0.5 h, 4 h, and 24 h, respectively. Increased [Ca2+]i in BP groups showed time-dependent relationship and the highest level in the 24 h group. There was positive correlation (0.5 h, 4 h) and no correlation (24 h) between [Ca2+]i and WC or EB. It is suggested that [Ca2+]i accumulation of cerebral neuronal cells plays an important role in the pathogenetic mechanism of the acute and delayed IBI induced by BP.