Conventional cytogenetics alone is not sufficient for identifying interspecies cell line contamination
Multani, A.S.; Pathak, S.
Anticancer Research 19(3a): 1753-1754
1999
ISSN/ISBN: 0250-7005 PMID: 10470110 Document Number: 507158
Evidence from our laboratory suggests that conventional chromosome banding analysis alone is not sufficient for detecting interspecies contamination of cell lines. To differentiate noncycling interphase cells of murine or other species origin that may be contaminating a human cell line, molecular cytogenetic techniques such as fluorescence in situ hybridization (FISH) with a total human DNA probe must be used.
Document emailed within 1 workday
Related Documents
Osterhaus, A. 2000: Circulation of virus and interspecies contamination in wild animals Bulletin de la Societe de Pathologie Exotique 93(3): 156Polianskaia, G.G.; Efremova, T.N. 1993: The effect of Mycoplasma contamination and decontamination with ciprofloxacin on the karyotypic structure of the Chinese hamster V-79 lung cell line Tsitologiia 35(8): 71-78
Solé, F.; Espinet, B.; Woessner, S. 1998: Priority of using conventional cytogenetics, in situ hybridization and PCR in the study of hematologic neoplasms Sangre 43(1): 3-5
Sugimoto, T.; Tatsumi, E.; Takeda, K.; Minato, K.; Sagawa, K.; Minowada, J. 1984: Modulation of cell surface antigens induced by 12-O-tetradecanoyl-phorbol 13-acetate in two myeloblastic cell lines, a promyelocytic cell line, and a monoblastic cell line: detection with five monoclonal antibodies Journal of the National Cancer Institute 72(4): 923-928
Bauer, S.R.; Holmes, K.L.; Morse, H.C.; Potter, M. 1986: Clonal relationship of the lymphoblastic cell line P388 to the macrophage cell line P388D1 as evidenced by immunoglobulin gene rearrangements and expression of cell surface antigens Journal of Immunology 136(12): 4695-4699
Gustafsson, B.; Hinkula, J. 1994: Antibody production of a human EBV-transformed B cell line and its heterohybridoma and trioma cell line descendants in different culture systems Human Antibodies and Hybridomas 5(3-4): 98-104
Beliaev, I.I.; Gracheva, M.P.; Obraztsov, N.V. 1982: Experience in identifying the source of benzo(a)pyrene contamination of the finished product in polyamine manufacture Gigiena i Sanitariia 7: 81
Obukhova, T.N.; Lorie, I.Iu.; Vodinskaia, L.A.; Alimova, G.A.; Nikitin, E.A.; Samoĭlova, R.S.; Kaplanskaia, I.B.; Domracheva, E.V. 2004: Cytogenetics of mantle cell lymphoma Terapevticheskii Arkhiv 76(7): 70-77
Ter-Grigorov, V.S.; Graf, I.A.; Ievleva, E.S.; Pivnik, A.V.; Idel'son, L.I. 1980: Antibodies against interspecies erythrokaryocyte antigen in patients with partial red cell aplasia of the bone marrow Biulleten' Eksperimental'noi Biologii i Meditsiny 90(10): 59-61
Akatsuka, A.; Shimamura, K.; Katoh, Y.; Takekoshi, S.; Nakamura, M.; Nomura, H.; Hasegawa, M.; Ueyama, Y.; Tamaoki, N. 1991: Electron microscopic identification of the intracellular secretion pathway of human G-CSF in a human tumor cell line: a comparative study with a Chinese hamster ovary cell line (IA1-7) transfected with human G-CSF cDNA Experimental Hematology 19(8): 768-772
Sorodoc, Y.; Cernescu, C.; Cajal, N. 1978: Persistent measles virus infection in a calf kidney cell line. Note II. Study of some biological markers of the measles virus isolated from a selected chronically infected cell line Virologie 29(2): 141-147
Francke, U. 1977: Cytogenetics and somatic cell genetics: the impact of chromosome banding Birth Defects Original Article Series 13(6): 79-103
Nozawa, S.; Tsukazaki, K.; Sakayori, M.; Jeng, C.H.; Iizuka, R. 1988: Establishment of a human ovarian clear cell carcinoma cell line (RMG-I) and its single cell cloning--with special reference to the stem cell of the tumor Human Cell 1(4): 426-435
Okoń, K.; Klimkowska, A.; Pawelec, A.; Dobrowolski, Z.; Kohla, Z.; Stachura, J. 2007: Immunophenotype and cytogenetics of mucinous tubular and spindle cell carcinoma of the kidney Polish Journal of Pathology: Official Journal of the Polish Society of Pathologists 58(4): 227-233
Federico, M.; Clò, V.; Carella, A.M. 1996: High-dose therapy autologous stem cell transplantation vs conventional therapy for patients with advanced Hodgkin's disease responding to first-line therapy: analysis of clinical characteristics of 51 patients enrolled in the HD01 protocol. EBMT/ANZLG/Intergroup HD01 Trial Leukemia 10 Suppl. 2: S69-S71
Jarrard, D.F.; Hansen, N.M.; Patai, B.; Rukstalis, D.B. 1995: Urokinase plasminogen activator is necessary but not sufficient for prostate cancer cell invasion Invasion and Metastasis 15(1-2): 34-45
Fogh, J.; Wright, W.C.; Loveless, J.D. 1977: Absence of HeLa cell contamination in 169 cell lines derived from human tumors Journal of the National Cancer Institute 58(2): 209-214
Gouy, H.; Deterre, P.; Debré, P.; Bismuth, G. 1994: Cell calcium signaling via GM1 cell surface gangliosides in the human Jurkat T cell line Journal of Immunology 152(7): 3271-3281
Hayakawa, M.; Osawa, A.; Nagakura, K.; Kikuchi, H. 1985: Heterogeneity of a KU-2 cell line derived from human renal cell carcinoma--differential susceptibilities of its sublines to NK cell-mediated cytotoxicity Hinyokika Kiyo. Acta Urologica Japonica 31(5): 763-768
Ristic-Fira, A.; Nikolic, D.; Petrovic, I.; Ruzdijic, S.; Raffaele, L.; Sabini, M.G.; Cirrone, G.A.; Cuttone, G.; Farruggia, G.; Masotti, L.; Kanazir, D.T. 2001: The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line Journal of Experimental and Clinical Cancer Research: Cr 20(1): 135-143