Change of high density lipoprotein receptor of hepatocyte during cholesterol gallstone formation in rabbit model
Shu, Y.; Xiao, L.; Zhao, J.; Zhu, H.; Zhou, Z.; Cheng, N.
Hua Xi Yi Ke da Xue Xue Bao 30(3): 296-298
1999
ISSN/ISBN: 0257-7712 PMID: 12212288 Document Number: 504122
In order to study the formation of cholesterol gallstone through rabbit model which was induced by high cholesterol diet (HCD), we investigated the high density lipoprotein receptor (HDLr) activities of hepatocytes, total bile acids and cholesterol of common duct bile at 1 week(1 w), 2 weeks(2 w), 3 weeks (3 w) and 4 weeks(4 w) in comparison with those of a control group respectively. The results were as follows: 1. The HDLr Bmax decreased and the kd value increased significantly (1 w group vs control group, P < 0.05); In 2 w group the two variables recovered to the level of control group; and after that, the HDLr Bmax increased and Kd value decreased markedly (3 w and 4 w groups vs control group, P < 0.05). 2. Total bile acids in bile increased slightly in 1 w group (vs control group, P > 0.05), then decreased in 2 w, 3 w and 4 w groups (4 w group vs control group, P < 0.05). 3. The concentration of cholesterol in bile increased gradually as the time of feeding HCD went on (3 w and 4 w groups vs control group, P < 0.05). The results demonstrated that owing to the intake of HCD with the passage of time, the activity of HDLr was up at the beginning and down gradually in the rest period. The change in concentrations of bile acids was similar to that of HDLr, and the concentration of cholesterol in bile was foreign to the change of HDLr. These results suggest that the decrease of HDLr activity may cause the absence of material for bile acids synthesis and it may play an important role in the formation of gallstone.