Experimental study of the pathogenesis of frostbite. Part II. Role of the sympathetic-adrenergic system in tissue reperfusion immediately after thawing
Ilić, S.; Spaić, R.; Savić, J.; Spasić, P.; Skaro-Milić, A.; Cernak, I.; Milosavljević, I.
Vojnosanitetski Pregled 56(3): 249-254
1999
ISSN/ISBN: 0042-8450 PMID: 10518443 Document Number: 502893
The authors have investigated the role of sympathetic-adrenergic system in reperfusion of muscular tissue of the feet of Wistar rats immediately after thawing using alpha adrenergic blocker phentolamine methanesulfonate (Regitin). Cryoinjury was applied over an experimental model of local, controlled freezing of different intensity for certain experimental groups. Blood flow through microcirculation was measured by scintigraphy, following Tc-99m-pertechnetate clearance that was given in muscular mass of frozen right, as well as unfrozen left feet of rats. Results obtained in some experimental groups were compared mutually, as well as with the control group that was not exposed to cryoinjury. The investigations have revealed that immediately after thawing existed significant reduction, non-dependent of the intensity of freezing, in blood flow in microcirculation of both frozen and unfrozen feet of rats compared to the control group. Significant increase of microcirculatory blood flow in unfrozen feet was caused by blockade of sympathetic-adrenergic system, while the blood flow remained unchanged in the frozen ones. The reduction of blood flow in microcirculation of frozen tissues immediately after thawing was not associated with sympathetic-adrenergic factor.