Surface changes and hormone production in pituitary cells during tumorigenesis
Khalil, W.K.; Gáspár, L.; Szalai, G.; Julesz, J.; Galfi, M.
Journal of Experimental and Clinical Cancer Research Cr 18(4): 553-558
1999
ISSN/ISBN: 0392-9078 PMID: 10746984 Document Number: 501228
Prolactinomas were induced by chronic estrone acetate treatment in female Wistar rats in vivo. After enzymatic dissociation of the tumor tissue, monolayer cell cultures were obtained. In vitro tumor induction was performed by treatment of normal monolayer anterior pituitary cell cultures with a 1:1 mixture of 7,9-dimethylbenzacridine treatment were able to attach only after ammonia activation of the collagen surface. These findings strongly suggest that the mode of tumor induction can result in differences in membrane fluidity; this phenomenon is possibly connected with the levels of prolactin, adrenocorticotropin and alpha-melanotropin hormone production of these endocrine tumor cells.