PCR-based DNA analysis of vWA31A, TH01, F13A01, FESFPS, TPOX and CSF1PO
Jiang, B.; Guo, J.Y.; Liang, S.Q.
Fa Yi Xue Za Zhi 15(3): 141-143; 190
1999
ISSN/ISBN: 1004-5619 PMID: 12536445 Document Number: 499957
The allele and genotype frequencies of 6 tetranucleotide STR loci were investigated in a sample of 132 unrelated individuals from Chinese Han population. The PCR products were analyzed on 6% denaturing PAGE and detected using fluorescently labeled primers in an automated 377 sequencer(PE). All loci meet Hardy-Weinberg equilibrium. There was no random association of alleles among the 6 loci. The allele frequencies were compared with other population databases. Except locus vWA31A, the observed heterozygosity at other 5 loci was significantly lower than that reported in Caucasian and Black population studies. The calculated DP = 0.99999, PE = 0.9708, pM = 1.059 x 10(-5). The allelic frequency data can be used in forensic identification and paternity testing.