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Production of recombinant hIL-4delta2-a native isoform of human interleukin-4 in Escherichia coli cells

Ptitsyn, L.R.; Smirnov, S.V.; Al'tman, I.B.; Samsonova, N.N.; Khodiakova, A.V.; Vasilenko, R.N.

Bioorganicheskaia Khimiia 25(8): 623-629

1999

ISSN/ISBN: 0132-3423
PMID: 10578467
Document Number: 499381
Expression plasmids containing the synthetic gene hil-4 delta 2 was constructed to produce human interleukin-4 in Escherichia coli cells. Strains TG1 (pBTIL-4 delta 2) and BL21 (DE3) (pETIL-4 delta 2) produced the recombinant protein as inclusion bodies, and its production level was up to 30% of the total cell protein. The renatured hIL-4 delta 2 inhibited IL-4-stimulated T cell proliferation, and this effect was enhanced by cyclosporin A.

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