Effect of recombinant human fibronectin polypeptide CH50 on growth and metastasis of melanoma
Fan, Q.; Feng, Z.H.; Zhang, G.M.; Zhang, H.; Cao, H.Q.
Zhongguo Yao Li Xue Bao 20(2): 175-178
1999
ISSN/ISBN: 0253-9756 PMID: 10437168 Document Number: 499161
AIM: To study the effect of recombinant human fibronectin polypeptide CH50 on murine melanoma growth and metastasis, and its antitumor mechanism. METHODS: Mouse and melanoma B16 cell tests were used to observe antitumor effect and mechanism of CH50. RESULTS: CH50 markedly inhibited melanoma growth and experimental lung metastasis. The melanoma weight was reduced from (2.3 +- 1.2) g of control group to (0.7 +- 0.8) g of test group (P < 0.05). CH50 at 0.125, 0.25, 0.5 mg/mouse reduced melanoma lung metastatic colonies from 87 +- 49 of control group to 34 +- 6, 14 +- 12, 4 +- 2, respectively. CH50 adhered to melanoma B16 cells and inhibited adhesion of B16 cells to laminin. CH50 enhanced the cytotoxicity of macrophages to melanoma B16 cells. CONCLUSION: CH50 inhibited tumor growth and metastasis of murine melanoma. The antitumor effect of CH50 is related to its adhesion ability to melanoma B16 cells and enhancing macrophage cytotoxicity.