Induction of quinone reductase and glutathion-s-transferase by dimethyl fumarate in rats
Wang, L.; Lin, S.
Zhonghua Yu Fang Yi Xue Za Zhi 33(6): 366-368
1999
ISSN/ISBN: 0253-9624 PMID: 11864513 Document Number: 498391
To assess induction of quinone reductase (QR) and glutathion-s-transferases (GSTs) by dimethyl fumarate (DMF) in major viscera of rats. Fodder fed to male Wistar rats was supplemented with 2.0% DMF, and blood specimens were collected form the rats 6 h, 24 h, 72 h, 1 week and 2 weeks after feeding, respectively, and then animals were killed. QR and GSTs activities were determined in rats' gland stomach, liver, lung, kidney and heart, and also QR, LDH and GPT activities in their sera were measured with enzyme dynamic methods. Administration of DMF for 24 h, 72 h, 1 week and 2 weeks in rats can significantly increase activities of QR and GSTs in their gland stomach, liver, lung and kidney, as compared with those in controls (P < 0.0001, or P < 0.01), and their serum QR also significantly increased (P < 0.0001, or P < 0.01). But, DMF had no effect on activities of serum GPT and LDH (P > 0.05). DMF is a potent induction agent for QR and GSTs in the gland stomach, liver, lung and kidney of rats, which suggests it will hopefully be a protective and antidotic agent against human antioxidant injury.