Treatment of avascular necrosis of femoral head by periosteal cell transplantation: an experimental study
Song, S.; Zhu, S.; Sun, C.
Zhonghua Yi Xue Za Zhi 78(1): 52-55
1998
ISSN/ISBN: 0376-2491 PMID: 10923406 Document Number: 497433
To investigate the effect of periosteal cell transplantation on the repair of avascular necrosis of femoral head (ANFH). On the basis of successful culture of periosteal cells in vitro. The tibial periostea of new born dog were excised and cultured under aseptic conditions. Eight adult dogs were used and divided into 2 groups: experimental group and control group. The animal model of ANFH was made by transferring the freed femoral head into the muscle under the ilium on one side in each dog. Two weeks after the establishment of ANFH, the cultured periosteal cells were collected and transplanted into the necrotic femoral heads in the experimental group, and the femoral heads without periosteal cell transplantation served as the control. Six weeks after cell transplantation, the femoral heads were taken out and histological examination was made. There was active bone formation in the experimental group. The new bone was arranged into bone trabeculae, most of which were covered by a monolayer of osteoblasts. Between bone trabeculae were fibrous tissue rich in blood vessels. In the control group, the bone trabeculae withered and remained necrotic. There were fibrous tissues with few blood vessels between bone trabeculae. Periosteal cell transplantation can improve the repair of ANFH.