Top Abstract Purchase Related
Home
>
Node
>
Subnode

Application of the PRINS technique for chromosome examination in fetal cells present in maternal blood

Orsetti, B.; Lefort, G.; Boulot, P.; Andreo, B.; Pellestor, F.

Annales de Pathologie 18(5): 377-384

1998

ISSN/ISBN: 0242-6498
PMID: 9864573
Document Number: 497177
Both detection and chromosomal analysis of fetal cells present in the maternal circulation can be performed using Histopaque double density gradient centrifugation followed by primed in situ (PRINS) labeling technique. This approach has been tested on blood samples from 15 pregnant women and 6 control donors with primers specific for chromosomes 9, X and Y. The cell separation technique allows recovery of both mononuclear cells and polynuclear cells with a 97% efficiency. PRINS labeling was successful in 100% cells from control blood samples. Among patient samples, 2 "false-negative" results were observed. These preliminary results suggest that the present protocol might be efficient for non-invasive prenatal chromosome analysis.

Document emailed within 1 workday
Secure & encrypted payments

Zhou, Z. 1999: Progress in the technique of sorting fetal cells from maternal peripheral blood Zhonghua Fu Chan Ke Za Zhi 34(6): 373-375
Xiaoyan, X.; Hanping, C. 2004: Fetal nucleated red blood cells in maternal peripheral blood and gestational age International Journal of Gynaecology and Obstetrics: the Official Organ of the International Federation of Gynaecology and Obstetrics 87(2): 143-144
Daffos, F.; Forestier, F.; Nahoul, K.; Castanier, M.; Capella-Pavlovsky, M.; Chartier, M.; Scholler, R. 1984: Fetal hormonology by direct sampling of fetal blood during the 2d trimester of pregnancy. Study of fetal and maternal 5-ene steroid sulfates Pathologie-Biologie 32(8): 891-894
Hou, J.W.; Wang, T.R. 1997: Primed in situ (PRINS) labeling for rapid detection of numeric and structural chromosome anomalies Journal of the Formosan Medical Association 96(1): 46-50
Grabowska, A.; Madetko-Talowska, A.; Janeczko, M.; Majka, M.; Pietrzyk, J.J. 2011: Fetal CD34+ cells isolated from maternal blood and cytogenetics array as potential tools in screening, non-invasive prenatal diagnosis--preliminary research Przeglad Lekarski 68(1): 40-43
Song, E.Y.; Yoon, J.H.; Shin, S.; Chang, J.Y.; Hwang, K-Tae.; Roh, E.Y. 2019: Maternal lipid profiles vs. fetal growth and cord blood hematopoietic cells: weak associations in healthy Korean newborn-mother pairs Minerva pediatrica 2019
Lin, S.-K.; Ho, E.S.-C.; Hsieh, Y.-T.; Lo, F.-C.; Lai, H.-Y.; Chen, M.-H. 2002: Limited feasibility of routinely analyzing fetal cells from maternal blood by using magnetic activated cell sorting and polymerase chain reaction for prenatal diagnosis Zhonghua Yi Xue Za Zhi 65(12): 594-599
Liu, Y.-n.; Zhao, X.-y.; Ping, Y.; Xu, Q.-w.; Huang, J.-p.; Zou, K.-n.; Zhou, H.-g. 2015: Application of Ion Torrent PGM™ System in Detection of Fetal DNA in Maternal Plasma Fa Yi Xue Za Zhi 31(6): 432-435
Leng, B.; Leng, J.J.; Lesgouarres, M.T.; Dubecq, J.P. 1976: Concentration of metampicillin in the maternal blood, fetal blood and in the amniotic fluid Revue Francaise de Gynecologie et d'Obstetrique 71(12): 775-777
Ricard, M.P.; Muñoz, M.A.; López Rubio, M.; Arribalzaga, K. 1996: Rapid differential diagnosis of fetal blood versus maternal blood Sangre 41(5): 400-401
Moll, W. 1992: Blood flow velocity and Doppler shift in fetal and maternal blood vessels. Physiologic principles Der Gynakologe 25(5): 278-283
Laplace, M.; Boncour, R.; Lebbe, J.; Lafarge, J.P. 1971: Application of gas chromatography to the examination of blood volatile components. II. Determination of blood alcohol Archives des Maladies Professionnelles de Medecine du Travail et de Securite Sociale 32(3): 265-270
Gladkikh, A.S. 1970: Medico-legal significance of blood studies in determination of pregnancy, puerperium, and differences between maternal and fetal blood Sudebno-Meditsinskaia Ekspertiza 13(2): 25-29
Radecki, W. 1972: Beta-glucuronidase activity in the amniotic fluid, cord blood and maternal blood serum in the course of normal deliveries and those complicated by fetal hypoxia Ginekologia Polska 43(3): 313-316
Nagy, G.R.ár.; Bán, Z.án.; Sipos, F.; Fent, J.án.; Oroszné Nagy, J.; Beke, A.úr.; Furész, J.óz.; Papp, Z.án. 2004: First attempts of detecting fetal cells in the maternal circulation Orvosi Hetilap 145(44): 2231-2236
Rasmussen, J. 1971: Quantitative determination of fetal erythrocytes in maternal blood Nordisk Medicin 85(2): 65
Scarpelli, P.T.; Fersini, C.; Sensi, G.; Corti, C.; Croppi, E.; Romano, S.; Tarquini, B. 1984: The maternal 24-h blood pressure (BP) profile as a predictor of fetal distress Chronobiologia 11(2): 141-145
Donskova, L.V. 1972: Correlation of estrogens in the maternal and fetal blood at the end of pregnancy and labor Sovetskaia Meditsina 35(10): 20-24
Tzingounis, V.; Katsilambros, N.; Prevedourakis, C.; Papaevangelou, G. 1977: Insulin levels in the amniotic fluid and in maternal and fetal blood Journal of Reproductive Medicine 19(5): 259-261
Van der Maaten, M.J.; Hubbert, W.T.; Boothe, A.D.; Bryner, J.H.; Estes, P.C. 1973: Isolations of bovine syncytial virus from maternal and fetal blood American Journal of Veterinary Research 34(3): 341-343