Effects of retionoic acid and GM-CSF on apoptosis of HL-60 cell induced by mild heat
Tan, M.; Qing, K.; Jiang, D.; Huang, Y.; Wang, Q.
Hunan Yi Ke da Xue Xue Bao 23(6): 524-526
1998
ISSN/ISBN: 1000-5625 PMID: 10806757 Document Number: 494041
The effects of several factors on the apoptosis of HL-60 cells, a human acute myeloid leukemia cell line, were investigated. The apoptosis model was established with mild heat. Typical changes of cell morphology and characteristic DNA ladder pattern were found. The effect of apoptosis induced by mild heat was enhanced, when HL-60 cells were pretreated with the retinoic acid. By contraries, that of apoptosis was displayed with the slight inhibition while HL-60 cells were pretreated with rhGM-CSF. We also observed the alterations of mRNA expressions of c-myc by the dot hybridization. The results indicate that both the mild heat and the combination of mild heat and retinoic acid obviously reduce the mRNA expression of c-myc compared to the control group.