Hyaluronic acid-complement interactions--I. Reversible heat-induced anticomplementary activity
Chang, N.S.; Boackle, R.J.; Armand, G.
Molecular Immunology 22(4): 391-397
1985
ISSN/ISBN: 0161-5890 PMID: 4033663 Document Number: 265633
The in vitro interaction of C1 as well as classical-pathway-mediated C3 conversion. However, if heat-treated HA samples are not stabilized but, rather, slowly cooled after heating or if heated HA samples are snapfrozen and then slowly thawed, the anticomplementary activity is gradually lost. The activity of this same sample can be regenerated by retreatment of the same sample with heat followed by low-temp stabilization, indicating the reversibility of the physical state of HA responsible for the anticomplementary effect. Since no detectable molecular degradation of thermally-treated HA was found, it was assumed that a heat-induced physical transition of HA (decoupled state) was responsible for the C-inhibitory effect.