Top Abstract Purchase Related
Home
>
Node
>
Subnode

Synthesis of new chromogenic substrates for aspartyl proteases

Litvinova, O.V.; Balandina, G.N.; Stepanov, V.M.

Bioorganicheskaia Khimiia 24(1): 10-15

1998

ISSN/ISBN: 0132-3423
PMID: 9551195
Document Number: 493831
A general method was developed for the synthesis of new chromogenic substrates of aspartyl proteases: Dnp-Ala-Xaa-Phe-Phe-Ala-Arg-NH2, Where Xaa was Ala or Ser. The synthetic scheme involved both chemical and enzymic stages, the condensation of tripeptides in an organic medium by means of pepsin immobilized on Celite being among the latters. The influence of organic solvents, reaction time, and the composition and ionic strength of the buffers used in the reaction mixture and at the pepsin immobilization step on the efficacy of the pepsin-catalyzed synthesis was studied.

Document emailed within 1 workday
Secure & encrypted payments

Juliano, M.A.; Juliano, L.; Biondi, L.; Rocchi, R. 1991: Synthesis and hydrolysis by arginyl-hydrolases of p-nitroanilide chromogenic substrates containing polyethylene glycol and D-gluconyl moieties Peptide Research 4(6): 334-339
Brand, J.A.; Tsang, V.C.; Zhou, W.; Shukla, S.B. 1990: Comparison of particulate 3,3',5,5'-tetramethylbenzidine and 3,3'-diaminobenzidine as chromogenic substrates for immunoblot Biotechniques 8(1): 58-60
Christensen, U. 1980: Requirements for valid assays of clotting enzymes using chromogenic substrates Thrombosis and Haemostasis 43(2): 169-174
Martin, C.; Orenga, S.; Doleans, F.; Denis, F. 1995: Value of media containing chromogenic substrates for the identification and the listing of urinary bacteria Pathologie-Biologie 43(9): 749-753
Chmielewska, J.; Latallo, Z.S. 1982: Experience with chromogenic substrates in studies of clotting and fibrinolytic systems Folia Haematologica 109(1): 111-114
Fukuda, C.; Iijima, K.; Nakamura, K.; Kasagi, T. 1986: Studies on urinary procoagulant assay method with chromogenic substrates (S-2222) Rinsho Byori. Japanese Journal of Clinical Pathology 34(2): 185-189
Bol'shakova, T.D.; Iakunin, G.A.; Smolianitskiĭ, A.Ia. 1985: Chromogenic substrates for determining coagulation factor X and their use in clinical practice (review of the literature) Laboratornoe Delo 1985(11): 643-648
Sonder, S.A.; Fenton, J.W. 1986: Thrombin specificity with tripeptide chromogenic substrates: comparison of human and bovine thrombins with and without fibrinogen clotting activities Clinical Chemistry 32(6): 934-937
Claeson, G. 1994: Synthetic peptides and peptidomimetics as substrates and inhibitors of thrombin and other proteases in the blood coagulation system Blood Coagulation and Fibrinolysis: An International Journal in Haemostasis and Thrombosis 5(3): 411-436
Loriia, Z.K.; Briukner, B.; Egorov, N.S. 1977: Correlation between the synthesis of extracellular proteases and the synthesis of the red pigment prodigiosin in Serratia marcescens Mikrobiologiia 46(4): 647-650
Krieg, R.; Halbhuber, K.J.; Oehring, H. 2000: Novel chromogenic substrates with metal chelating properties for the histochemical detection of peroxidasic activity, derived from 3-amino-9-ethylcarbazole (AEC) and 3,6-diamino-9-ethylcarbazole Cellular and Molecular Biology 46(7): 1191-1212
Kam, C.M.; Vlasuk, G.P.; Smith, D.E.; Arcuri, K.E.; Powers, J.C. 1990: Thioester chromogenic substrates for human factor VIIa: substituted isocoumarins are inhibitors of factor VIIa and in vitro anticoagulants Thrombosis and Haemostasis 64(1): 133-137
Fiedurek, J.; Paszczyński, A.; Ilczuk, Z. 1985: The effect of proteases on the synthesis of glucoamylase by mutants of Aspergillus niger C Acta Microbiologica Polonica 34(1): 25-32
Bokarewa, M.I.; Hellstrand, K.; Tarkowski, A. 2002: Phosphorotioated oligonucleotides trigger synthesis of human coagulation serine proteases Thrombosis and Haemostasis 88(5): 788-793
Mikel'saar, P.C.; Vilu, R.O.; Lakht, T.I. 1982: Dependence of extracellular proteases synthesis on the growth phase of Pseudomonas fluorescens Mikrobiologiia 51(2): 212-215
Kaluniants, K.A.; Strel'nikova, L.I.; Shteĭn, I.V. 1979: Effect of sources of carbon, nitrogen, and phosphorus on the synthesis of proteases from Bacillus subtilis cultures Prikladnaia Biokhimiia i Mikrobiologiia 15(1): 57-62
Pirog, T.P.; Vysiatetskaia, N.V.; Korzh, I.V. 2007: Synthesis of the exopolysaccharide ethapolan on a mixture of energy-deficient growth substrates Mikrobiologiia 76(1): 32-38
Gololobov, M.I.; Borisov, I.L.; Shviadas, V.K. 1987: Peptide synthesis catalyzed by proteases. Analysis of a kinetic model for enzymes with acyl-enzyme mechanism of action Biokhimiia 52(4): 584-591
Heine, H. 1984: Proteases - proteases inhibitors: a local cellular information system Advances in Experimental Medicine and Biology 167: 121-127
Rauterberg, E.W.; Ritz, E. 1988: Bioincompatibility of dialysis membranes: factor H binding correlates inversely with complement activation indicating a local imbalance of involved proteases/anti-proteases Advances in Experimental Medicine and Biology 240: 365-375