Metabolic transformation of (-) -clausenamide in rat liver microsomes
Yao, Q.; Wang, M.
Yao Xue Xue Bao 33(4): 296-299
1998
ISSN/ISBN: 0513-4870 PMID: 11939071 Document Number: 493811
The metabolic transformation of (-)-clausenamide, isolated from the leaves of Clausena lansium (Lour.) Skeels, was studied in vitro with phenobarbital-induced rat liver microsomal incubate containing the NADPH-generating system. The constitution of the incubation system was optimized and a RP-HPLC-DAD method was developed for the on-line analysis of (-)-clausenamide and its metabolites. The major metabolites were isolated and purified by silica gel column chromatography, preparative TLC and HPLC and their structures were determined as 6-OH clausenamide (CM1) and 5-OH clausenamide (CM2) by 1HNMR and MS.