Mapping of Drosophila mutations using site-specific male recombination
Chen, B.; Chu, T.; Harms, E.; Gergen, J.P.; Strickland, S.
Genetics 149(1): 157-163
1998
ISSN/ISBN: 0016-6731 PMID: 9584093 Document Number: 493722
Although recombination does not usually occur in the male Drosophila germline, site-specific recombination can be induced at the ends of P elements. This finding suggested that male recombination could be used to map Drosophila mutations. In this article, we describe the general method and its application to the mapping of two EMS-induced female-sterile mutations, grauzone and cortex. Within two months, the grauzone gene was mapped relative to seven different P-element insertion sites, and cortex was mapped relative to 23 different P-elements. The results allowed us to map grauzone to a region of about 50 kb, and cortex distal to the chromosomal region 33E. These experiments demonstrate that P-element-induced site-specific male recombination is an efficient and general method to map Drosophila autosomal mutations.