Observation of cicatricial fibroblasts in culture and its biological properties
Huang, Y.; Ren, L.; Qin, Y.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 12(6): 332-335
1998
ISSN/ISBN: 1002-1892 PMID: 10437084 Document Number: 493643
In order to study the biological properties of fibroblasts isolated from different tissues. The fibroblasts from normal skin, hypertrophic scar and keloid were cultured, respectively, in vitro, and their morphologies and growth kinetics were compared. The results revealed that although fibroblasts in keloid were irregularly arranged, crisscross and overlapping with loss of polarization, there was no significant difference in the 3 groups so far the cellular morphology of fibroblast itself, cellular growth curve, cellular mitotic index, cloning efficiency and DNA content provided those cultures were in the same cellular density and culture conditions. It was concluded that fibroblasts isolated from culture of normal skin, hypertrophic scar and keloid in vitro showed no significant difference in morphology and growth kinetics, on the contrary, their biological behaviors were quite similar.