Nitric oxide synthase induction in lines of macrophages from different anatomical sites
Palleroni, A.V.; Hajos, S.; Wright, R.B.; Palleroni, N.J.
Cellular and Molecular Biology 44(3): 527-535
1998
ISSN/ISBN: 0145-5680 PMID: 9620450 Document Number: 493626
Induction of nitric oxide synthase (iNOS) and nitric oxide (NO) production have been demonstrated using three macrophage cell lines from different anatomical sites, which had been immortalized using a rapid and convenient procedure previously described. Lysis of tumor cells presumably was caused by NO accumulation in the supernatants of cultures of the three cell lines after induction with a mixture of recombinant murine interferon gamma (rMuIFNgamma) and lipopolysaccharide (LPS). Induction by these two biological response modifiers (BRM) caused lysis of tumor cells and was repressed by addition of 1 microM (final concentration) of methyl-L-arginine (MMA) to the mixture during induction of the enzyme. Research using readily generated macrophage cell lines may facilitate clarify basic aspects of iNOS induction in these cells.