Kinetics of in vitro clonal growth of the murine MB-49 bladder tumor cell line
Páez Borda, A.; Angulo Frutos, J.; Luján Galán, M.; Llanes González, L.; Berenguer Sánchez, A.
Archivos Espanoles de Urologia 51(4): 319-324
1998
ISSN/ISBN: 0004-0614 PMID: 9656551 Document Number: 492386
To analyze the in vitro growth kinetics of the MB-49 murine bladder tumor cell line. Cell proliferation and duplication time (DT) were determined by 595 nm absorbance analysis and hemocytometry with and without fetal calf serum (FCS). The 595 nm absorbance and the hemocytometer cell count correlated according to the linear equation of cell number = 595 nm absorbance x 334.110-8.795 (r = 0.98). Cell proliferation was significantly higher in cultures with FCS (p < 0.001). However, a significant increase in the cell count (p < 0.01) was observed after 24 hours in cultures with and without FCS. DT was 21 and 50 hours for cultures with and without FCS, respectively. This experiment confirms the nature of the tumor cells and permits establishing the precise growth conditions of the MB-49 cells in this laboratory.