Oropharyngeal candidiasis in AIDS patients from Abidjan (Ivory Coast) : antifungal susceptibilities and multilocus enzyme electrophoresis analysis of Candida albicans isolates
Nébavi, F.; Arnavielhe, S.; Le Guennec, R.; Ménan, E.; Kacou, A.; Combe, P.; Aoussi, E.; Mallié, M.; Koné, M.; Bastide, J.M.
Pathologie-Biologie 46(5): 307-314
1998
ISSN/ISBN: 0369-8114 PMID: 9769890 Document Number: 491577
Multilocus enzyme electrophoresis (MEE) and in vitro antifungal susceptibility testing were used to investigate the C. albicans strain diversity in 29 AIDS patients from Abidjan (Cote d'Ivoire). All patients were monitored for a first episode of oropharyngeal candidosis and were randomly clustered into 3 groups of therapy: ketoconazole, amphotericin B or nystatin. Oral swabs were collected before every treatment, 14 and 30 days after the initiation of the therapy; a total of 67 isolates were investigated. No resistant or less susceptible isolate to any antifungal agent was found despite the emergence of clinical relapses, mainly for patients treated with nystatin or amphotericin B. The MEE analysis revealed 27 different electrophoretic types (ETs). Genetic distances between ETs were statistically analysed and represented on a dendrogram. The 27 ETs clustered into 3 groups; in each group, ETs represented variants of the same strain. A segregation of the C. albicans isolates seemed to be as a function of the serotype.