An analysis of hepatitis G virus infection status in various cases of hepatitis in Guangdong area
Yao, C.; Zhou, Y.; Xie, D.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 12(4): 330-332
1998
ISSN/ISBN: 1003-9279 PMID: 12526346 Document Number: 488223
To understand hepatitis G virus(HGV) infection status in different clinical types of hepatitis, 721 cases with hepatitis were analyzed in this article. PT-PCR technique was applied to amplify HGV gene, and heat denaturation method was used to extract HCV-RNA from sera. After turning to cDNA by reverse transcription(PT), a nested amplification was conducted and a 238 bp specific fragment was obtained. In this way, serum specimens of 721 cases with hepatitis, were tested including 587 hospitalized cases. 80 cases were found to be HGV RNA positive, of which positive rate of patients with non A-E hepatitis was high, HCV and HBV + HCV came the second, chronic severe hepatitis and liver cirrhosis (LC) was the lower. Results of research showed that HGV infection is prevalent to a certain degree among patients with viral hepatitis in Guangdong area.