Nuclear-cytoplasmic distribution of MAP-kinase isoforms p42 and p44 after treatment with epidermal growth factors (EGF) of cells with different functional state of EGF receptor
Arnautov, A.M.; Nikol'skiĭ, N.N.
Tsitologiia 40(7): 633-638
1998
ISSN/ISBN: 0041-3771 PMID: 9793176 Document Number: 487968
Nuclear translocation of MAPK in cell lines, expressing normal and mutant forms of EGF-receptors (EGFR), was investigated. Using immunoblotting and immunofluorescence, EGF-induced MAPK transport was discovered in cell lines, expressing both normal receptor and one with deletion of major autophosphorylation sites. The dynamics of MAPK nuclear translocation in these cell lines was alike. Cells bearing EGFR with inactive tyrosine kinase showed no ability to EGF-dependent activation and nuclear translocation of MAPK. A suggestion is made that tyrosine kinase is needed for EGF-induced activation and nuclear import of MAPK.