Determination of skin concentrations of enrofloxacin in dogs with pyoderma
DeManuelle, T.C.; Ihrke, P.J.; Brandt, C.M.; Kass, P.H.; Vulliet, P.R.
American Journal of Veterinary Research 59(12): 1599-1604
1998
ISSN/ISBN: 0002-9645 PMID: 9858413 Document Number: 487018
Objective-To compare serum and skin concentrations of enrofloxacin in dogs with pyoderma with those of clinically normal dogs and to evaluate concentrations in dogs with superficial versus deep pyoderma. Animals-16 clinically normal dogs and 16 dogs with pyoderma. Procedure-Enrofloxacin (approx 5 mg/kg of body weight, PO) was administered daily to all dogs. Serum samples and skin biopsy specimens were obtained on day 1 at 3 hours after drug administration and on day 3 immediately before and 3 hours after drug administration. Samples and specimens were assayed by high-performance liquid chromatography. Morphometric analysis was performed on skin biopsy specimens to determine correlation between inflammatory cells and peak tissue enrofloxacin concentration on day 1. Results-Morphometric analysis revealed high correlation between dermal inflammatory cell count and drug concentration in dogs with pyoderma. Conclusions-At mean dosage of 5 mg/kg once daily, enrofloxacin tissue concentrations were significantly greater in dogs with pyoderma at 3 hours after pill administration. Enrofloxacin tissue concentration on day 3 at 3 hours after pill administration was 12.4 times the 90% minimum inhibitory concentration of enrofloxacin for Staphylococcus intermedius. Clinical Relevance-In dogs with pyoderma, therapeutic tissue concentrations of enrofloxacin are reached as early as 3 hours after drug administration.