Cloning and expression of porcine recombinant soluble tumor necrosis factor receptor 1
Maroushek Boury, N.; Bosworth, B.T.; Stabel, T.J.; Kehrli, M.E.; Taylor, M.J.
American Journal of Veterinary Research 59(10): 1317-1322
1998
ISSN/ISBN: 0002-9645 PMID: 9781468 Document Number: 486043
Objective-To clone, sequence, and express porcine recombinant soluble tumor necrosis factor receptor 1 (sTNFR1). Procedure-A polymerase chain reaction (PCR)based library enrichment technique was used to isolate a fragment of porcine TNFR1. The mature extracellular domain of porcine TNFR1 was subcloned into an expression vector and expressed in Escherichia coli as a fusion protein. Protein product was purified by immunoaffinity chromatography, using a commercially available affinity gel specific for the marker peptide of the fusion protein. The bioactivity of the purified protein was tested for its ability to inhibit TNF- mediated cytotoxicity in a PK(15) bioassay. encoding the entire extracellular and transmembrane domains, as well as 75 amino acids of the cytoplasmic domain, was isolated from a porcine lung cDNA library. The extracellular domain was expressed as a soluble TNFR1 fusion protein with a yield of 120 to 150 mug/L of culture, Affinity-purified porcine sTNFR1 was able to inhibit TNF-mediated cytotoxicity of porcine PK(15) cells in dose-dependent manner. Conclusions-Porcine recombinant sTNFR1 inhibits TNF bioactivity in vitro. This recombinant protein will be useful for developing TNFR1 antibodies and studying the roles of TNF and TNFR1 in the pathogenesis of infectious diseases in swine.