Chemical modification of L-asparaginase with comb-shaped copolymer of poly(ethylene glycol) derivative and maleic anhydride

Saito, T.; Ueno, T.; Sekine, T.; Kodera, Y.; Hiroto, M.; Matsushima, A.; Nishimura, H.; Inada, Y.; Saito, T; Ueno, T; Sekine, T; Kodera, Y; Hiroto, M; Matsushima, A; Nishimura, H; Inada, Y

Home

>

Node

>

Subnode

Chemical modification of L-asparaginase with comb-shaped copolymer of poly (ethylene glycol) derivative and maleic anhydride

Saito, T.; Ueno, T.; Sekine, T.; Kodera, Y.; Hiroto, M.; Matsushima, A.; Nishimura, H.; Inada, Y.

Leukemia 11(Suppl 3): 408-409

1997

ISSN/ISBN: 0887-6924

PMID: 9209407

Document Number: 482786

L-asparaginase from Escherichia coli, an antitumor enzyme, was chemically modified with a comb-shaped copolymer of poly(ethylene glycol) derivative and maleic anhydride (activated PM). The PM-modified asparaginase lost the immunoreactivity with retaining high enzymic activity and also prolonged the clearance time in blood. Intraperitoneal administration of PM-asparaginase markedly increased the mean survival-time of lymphoma L5178Y-bearing mice in comparison with that of unmodified asparaginase. Pretreatment of mice with PM-asparaginase before immunizing with unmodified asparaginase extremely suppressed the anti-asparaginase antibody production.