Effect of various compounds on electrophoretic spectra of multiple molecular forms of peroxidase
Toptikov, V.A.; D'iachenko, L.F.; Totskiĭ, V.N.
Ukrainskii Biokhimicheskii Zhurnal 69(1): 41-49
1997
ISSN/ISBN: 0201-8470 PMID: 9454377 Document Number: 482389
The effect of different reagents to colour intensity on PAA gel bands using benzidine was studied. All the investigated reagents were divided into three groups 1) reagents with no effect on visualization of peroxidase molecular forms colour intensity; 2) those relatively equally decreasing colour intensity; 3) selective effect on visualization of peroxidase molecular forms. Ferulic, ascorbic and indole-3-acetic acids, phloroglucin and hydroquinone are most interesting in our investigation. Use of these reagents allows increasing the differences between electrophoretic pictures during comparative physiological and genetic experiments. Possible mechanisms of the mentioned reagents influence on efficiency of peroxidase benzidine interaction are discussed.