Development of new cell fusion technique by laser device and application to bio-medical field
Itagaki, H.; Doi, H.; Ohkohchi, N.; Satomi, S.
Nihon Rinsho. Japanese Journal of Clinical Medicine 55(10): 2780-2787
1997
ISSN/ISBN: 0047-1852 PMID: 9360406 Document Number: 482173
We developed a new cell fusion method which was sterile, noncontact, and selective technique under the microscope, using the micro-processing device by LASER. By this technique, we succeeded in fusing myeloma cell (SP2) and lymphocyte in mouse. We also defined the proliferation of the fused cells in HAT medium and the function of the fused cells in the Ouchterlony method, i.e. production of IgG. This method enable us to make hybridomas from very small number of cells and to fuse target cells selectively. This method is applicable to fuse cells which are difficult to be fused by conventional methods.