Proliferative activity of lymphocytes in a culture with phytohemagglutinin and immunity parameters in acute pneumonia
Shmarov, D.A.; Nikulin, B.A.; Kozinets, G.I.
Klinicheskaia Laboratornaia Diagnostika 1997(4): 36-39
1997
ISSN/ISBN: 0869-2084 PMID: 9221076 Document Number: 480629
Proliferative activity of lymphocytes in a culture was assessed by flow cytometry and lymphocyte blast transformation test (LBTT) with the proliferative pool of cells (S+G2+M) from 150 patients with acute pneumonia, 30 of these with the mild form, 97 with medium-severe, and 27 with grave form; in 43 patients the disease duration was over 30 days (protracted pneumonia). In addition, levels of R-protein, homoreactant, immunoglobulins, complement components, T, B, and active T lymphocytes, phagocytosis, and granulocyte oxidative metabolism were assessed. LBTT values were in inverse relationship with the disease severity. Correlations between the studied immunity parameters were analyzed. The findings indicate that LBTT is an informative method for assessing the immune reactivity and may serve for predicting the disease course and for the differential diagnosis of pulmonary diseases.