Effects of Tremella polysaccharide on cytoplasmic free calcium concentration in murine splenocytes
Cui, J.Y.; Lin, Z.B.
Yao Xue Xue Bao 32(8): 561-564
1997
ISSN/ISBN: 0513-4870 PMID: 11596301 Document Number: 480207
Effects of Tremella polysaccharides (TP) isolated from Tremella fuciformis Berk. on the cytoplasmic free calcium concentration in murine splenocytes were observed using Fura-2, a fluorescence indicator, technique. TP increased the cytoplasmic free calcium concentration of Fura-2-loaded murine splenocytes in a concentration-dependent manner and showed synergetic effect with Con A. No effect was found in the release of intracellular calcium without extracellular calcium. Verapamil (10 mug cntdot ml-1), a calcium channel blocker, was shown to inhibit the effect of TP on cytoplasmic free calcium, indicating that TP can affect the influx of extracellular calcium.