Chemical characterization of avian infectious bronchitis virus receptor sites
Lukert, P.D.
American Journal of Veterinary Research 33(5): 987-994
1972
ISSN/ISBN: 0002-9645 PMID: 5023057 Document Number: 47904
The attachment of avian infectious bronchitis virus (IBV) to chick-embryo kidney cell monolayers was inhibited by sulfhydryl (SH) compounds such as L-cysteine, reduced glutathione, 1,4-dithiothreitol (DTT), and 2-mercaptoethanol. Virus inactivated by the SH reagent p-hydroxymercuribenzoate (PHMB) was reactivated with L-cysteine. Viral attachment sites on both the virion and the cell were inactivated by PHMB treatment. The cellular receptors of IBV were also susceptible to neuraminidase, as were those of Newcastle disease virus (NDV), and both IBV and NDV attachments were inhibited in the presence of N-acetylneuraminic acid. The attachment of Newcastle disease virus was not affected by SH compounds nor was the virus inactivated by PHMB. Salicylates did not have an effect on IBV attachment, but pancreatin enhanced the attachment of IBV to chick-embryo kidney cells. Cellular receptors for IBV are apparently complex and contain SH groups and N-acetylneuramic acid as integral parts of the receptor site. The complexity of receptor sites may be used to explain why certain viral inactivants do not completely inactivate a viral preparation by a first-order kinetic reaction.