Activation of L-Arg: no pathway in canine brain by the damage from complete cerebral ischemia-reperfusion
Cheng, Z.; Cai, H.; Guo, Q.; Ou, Y.; Huan, J.; Tan, X.; Xu, Q.
Hunan Yi Ke da Xue Xue Bao 22(3): 192-194
1997
ISSN/ISBN: 1000-5625 PMID: 9868109 Document Number: 477662
To ascertain whether complete cerebral ischemia-reperfusion activate L-Arg: NO pathway in canine brain, we anestherized nine adult dogs with ketamine and fentayle and randomly divided into two groups. Four dogs were nonischemic control group. Five dogs were complete cerebral ischemia-reperfusion group, they underwent a 18-minute cardiac arrest, and were resusciatation by standard CPR, supported by intensive care for 8 hours. At the end of each experiment, the parietal cortex was assayed for content of Nitrite and NADPH-positive neurons. Compared with the control group, the contents of Nitrite and NADPH-positive neurons of coxtex in complete cerebral ischemia-reperfusion group increased significantly (P < 0.01). The results suggest that complete cerebral ischemia-reperfusion activate the L-Arg: NO pathway in canine brain, and NO may play an important role in cerebral ischemia-reperfusion injury.