Designing of plant artificial chromosome (PAC) by using the Chlorella smallest chromosome as a model system
Noutoshi, Y.; Arai, R.; Fujie, M.; Yamada, T.
Nucleic Acids Symposium Series 37: 143-144
1997
ISSN/ISBN: 0261-3166 PMID: 9586040 Document Number: 476538
As a model for plant-type chromosomes, we have been characterizing molecular organization of the Chlorella vulgaris C-169 chromosome I. To identify chromosome structural elements including the centromeric region and replication origins, we constructed a chromosome I specific cosmid library and aligned each cosmid clones to generate contigs. So far, more than 80% of the entire chromosome I has been covered. A complete clonal physical reconstitution of chromosome I provides information on the structure and genomic organization of plant genome. We propose our strategy to construct an artificial chromosome by assembling the functional chromosome structural elements identified on Chrorella chromosome I.